DMV at CU examines a various aspects of the community of microorganisms – biofilm, such as communication, adaptation to adverse conditions, the immune-modulatory properties of surface antigens and their role in the adherence to biotic and abiotic surfaces. The main focus covers investigation of biofilm formed by different Candida spp., namely Candida albicans, Candida glabrata, Candida parapsilosis, and a new pathogen of Candida auris together with bacteria, mainly with Staphylococcus aureus. Different methods of microbiology and molecular biology (CLSM, real time PCR, different antimicrobial assays), biocompatibility and cytotoxicity testing on ex vivo (cell lines, i.e. HeLa cells) and in vivo model organism of Galleria mellonella are the part of the group expertise. A few years ago, the group involved a study of anti-biofilm properties of new materials based on clay minerals and photoactive compounds.

List of the most relevant references and projects (last 5 years)


Dadi NCT, Dohál M, Medvecká V, Bujdák J, Koči K, Zahoranová A, Bujdáková H. Physico-chemical characterization and antimicrobial properties of hybrid film based on saponite and phloxine B. Molecules. 2021 Jan 10;26(2):325. doi: 10.3390/molecules26020325.

Joao I, Bujdáková H, Jordao L. Opportunist coinfections by nontuberculous mycobacteria and fungi in immunocompromised patients. Antibiotics (Basel). 2020 Nov 2;9(11):771. doi: 10.3390/antibiotics9110771.

Ramstedt M, Ribeiro IAC, Bujdakova H, Mergulhão FJM, Jordao L, Thomsen P, Alm M, Burmølle M, Vladkova T, Can F, Reches M, Riool M, Barros A, Reis RL, Meaurio E, Kikhney J, Moter A, Zaat SAJ, SjollemaJ.EvaluatingEfficacyofAntimicrobial and AntifoulingMaterialsforUrinaryTractMedicalDevices: Challenges and Recommendations. MacromolBiosci. 2019 May;19(5):e1800384. doi: 10.1002/mabi.201800384. Epub 2019 Mar 18. Review

Gaalova B, Vyletelova I, Pokorna K, Kikhney J, Moter A, Bujdak J, Bujdakova H. Decreased vitality and viabilityofEscherichia coliisolates by adherence to saponite particles. AppliedClay Science. 2019 Vol.: 183; ArticleNumber: 105316. DOI: 10.1016/j.clay.2019.105316.

Černáková L, Dižová S, Gášková D, Jančíková I, Bujdáková H. Impactoffarnesol as a modulatorofeffluxpumps in a fluconazole-resistantstrainofCandidaalbicans. MicrobDrugResist. 2019 Jul/Aug;25(6):805-812. doi: 10.1089/mdr.2017.0332.

Bujdakova H.; Bujdakova, V.; Majekova-Koscova H.; Gaalova B.; Bizovska V.; Bohac P.; Bujdak J.: Antimicrobialactivityoforganoclaysbased on quaternaryalkylammonium and alkylphosphoniumsurfactants and montmorillonite. AppliedClay Science. 2019 Volume: 158; Pages: 21-28. DOI:10.1016/j.clay. 2018.03.010.

Dekkerová J, Lopez-Ribot JL, Bujdáková H. Activityof anti-CR3-RP polyclonal antibody against biofilms formed by Candida auris, a multidrug-resistant emerging fungal pathogen. Eur J Clin Microbiol Infect Dis. 2019 Jan;38(1):101-108. doi: 10.1007/s10096-018-3400-x. Epub 2018 Oct 16.

Černáková L, Jordao L, Bujdáková H. Impactoffarnesol and Corsodyl® on Candida albicans forming dual biofilm with Streptococcus mutans. Oral Dis. 2018 Sep;24(6):1126-1131. doi: 10.1111/odi.12873. Epub 2018 Jun 8.

Chupáčová J, Borghi E, Morace G, Los A, Bujdáková H. Anti-biofilm aktivity of antibody directed agains tsurface antigen complement receptor 3-related protein-comparison of Candida albicans and Candida dubliniensis. Pathog Dis. 2018 Feb 1;76(1). doi: 10.1093/femspd/ftx127.

Černáková L, Dižová S, Bujdáková H. Employment of methylene blue irradiated with laser light source in photodynamic inactivation of biofilm formed by Candida albicans strain resistant to fluconazole. Med Mycol. 2017 Oct 1;55(7):748-753. doi: 10.1093/mmy/myw137.

Domestic and international projects

Grant VEGA 1/0628/15 Single-species and polymicrobial biofilms formed by yeasts of the genus Candida and Gram-positive bacteria Staphylococcus aureus and Streptococcus mutans (01/2015 – 12/2018).

Grant VEGA 1/0537/19 Photodynamic inactivation–a unique approach in eradication of microbial biofilms (01/2019 – 12.2022)

Grant APVV-0291-11 (Photoactive hybrid nanomaterials with luminescent and antimicrobial properties) (07/2012 – 12/2015).

Grant APVV-15-0347 Prevention and eradication of microbial biofilms in relationship to nanomaterials (07/2016 – 06/2020).

Grant SK-PT-18-0006 Understanding biofilms associated with infections, (01/2019-12/2021, bilateral project with Instituto Nacional de SaúdeDoutor Ricardo Jorge, Portugal)

COST Action, TD1305 Improved Protection of Medical Devices against Infection (IPROMEDAI; 05/2014-04/2018 Management Committee Member)

WIDESPREAD-05-2020; EU project 952398 Excellence in the research area of Combating and evaluation of mixed biofilms. Horizon 2020 Grant Scheme; (12/2020-11/2023)